I run 10% TBE-UREA gel to visualize DNA:RNA hybrid and ssDNA (single stranded DNA). Goal of the experiemnt was to see enzyme digestion effect of enzymes S1 nuclease and Mung bean nuclease on DNA:RNA hybrid and ssDNA. 10 bp DNA ladder was used. Urea-buffer was added to all the samples and the ladder and was heated to 70 C for 3 mins before laoding on the gel.
PFA attached pictures of the run.
There are two bands in the first picture i wanted to know which band is DNA and which band is RNA? As both them are single stranded and same number of nucleotides which is 48 then why they form different bands ?
IF they do form different bands is the lower one DNA becasue RNA has higher mass to charge ratio and will travel slower.
The second gel is just ssDNA with and without enzyme treatment. Both gels were run simultaneously on life technologies mini gel tank. Bands look similar to RNA band in gel one but the size coressponds to DNA if you go by my reasoning !!
Can anyone help out ... i can give more details if that helps but i think i have mentioned all the information.