Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

qPCR acceptable standard deviation

qPCR deviation

  • Please log in to reply
1 reply to this topic

#1 Hidenori Okamura

Hidenori Okamura

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 03 March 2015 - 05:41 AM

Hello,

 

My doubt is: what is the acceptable standard deviation among my samples?

Sometimes my triplicates present a SD of 0,2 or 0,3. But sometimes this SD are 0,5 to 1,5. What value is acceptable?

Should I just eliminate one repetition to diminish my SD?

 

Thank you!



#2 5280

5280

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 122 posts
7
Neutral

Posted 05 March 2015 - 07:59 PM

For unknown samples, I prefer to look at my %CV then my standard deviation. I try to keep my %CV within 10% but sometimes will accept 15-20% among my replicates if the gene expression that I am measuring is very low where you are likely to see more variation between replicates. I only exclude a replicate if two of the replicates are tight and the third is off. I rarely exclude replicates so if you are having to exclude replicates often then you should try to improve the technical reproducibility and focus on your pipetting, mixing samples, etc. 







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.