Hi Good People,
I am designing a project to investigate the role of a signaling pathway/molecule in a type of cancer. I have isolated spheroid cells from mouse tissue and currently passaging. I'm thinking of doing ELISA with an antibody to the target molecule. Could you share some suggestions on how best to proceed. Thanks!
PS: I've no experience in experimental design, have always had to follow instructions now thrilled to have this opportunity to do it from scratch and looking forward to all your input!