
#1
Posted 02 March 2015 - 06:52 PM
#2
Posted 04 March 2015 - 01:31 PM
So you are looking to check that the excised product is no longer in the plasmid? If you did this, then I would assume that the plasmid is no longer circular, and as such won't give you a product if you are trying to amplify across the vector ends.
If you are thinking that the transposon is excised after transcription, then you will definitely have to extract RNA, make cDNA and the PCR.
However, assuming that the mechanism is correct, that GFP can only be expressed when the transposon is excised, then surely GFP expression is all you need to see (though RNA would confirm it).
Also tagged with one or more of these keywords: PCR, mammalian cell
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