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Reference Gene/HKG- every plate

qpcr reference gene

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#1 ryc

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Posted 02 March 2015 - 03:50 PM

Hi,

 

Is it necessary to run reference genes/housekeeping genes on each plate? For example is would it be okay to run the reference genes on plate 1 and then compare target genes run on plates 2 & 3.

 

Thank You



#2 bob1

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Posted 04 March 2015 - 01:25 PM

You would need an inter-plate comparator to do this, otherwise you should run your HKG on every plate.







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