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Dislodge cell in 96well plate

cell culture

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#1 ahmadfathi

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Posted 01 March 2015 - 06:38 PM

Hi,

 

Right now i doing expression using 96 well plate but face the problem how to dislodge all of my cell from the plate for harvesting purpose. Until now i just pipette up and down several times but not all my cell were dislodged. Hopefully somebody can help me?

 

 



#2 SusieQ

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Posted 09 March 2015 - 06:47 AM

Do you mean to lyse them? If so, adding a soapy buffer could help, like a Triton-solution. This will dissolve your cells.







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