I am having some issues getting good resolution bands from my PCR. Originally my mistake was that my dNTP's were not dilute enough but I made new aliquots and started getting banding. My bands are not that bright though and I have tried many concentrations of DNA (Full strength; 1:5; 1:10; 1:30). I also tried messing with the dNTP dilutions (1:5; 1:6; 1:7; 1:8). I also am getting double banding which for what I've heard means that there is to much dNTP but the catch is that on my last few gels its been on the more diluted samples. I will post the picture of my most recent and probably my best gel. Any suggestions would be taken kindly! Thanks!