How to insert PreScission protease into a vector
Posted 25 February 2015 - 07:07 AM
Posted 14 March 2015 - 11:44 AM
Are you trying to fuse the entire prescion protease gene to your 15bp insert, or are you trying to fuse a precision protease cut site to your 15bp insert?
If the former, forget those silly expensive kits...use overlap extension pcr.
If the latter, encode the cut site in a primer along with your 15bp insert. Add restriction sites to primer at both ends to ligate into vector.
More info would be helpful.