Hello,
I have carried out gel electrophoresis for a construct that should be ~800bp. However, the DNA band spans from 750-950bp, instead of the usual thin line as the band. Why has this happened?
Thanks.
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PCR DNA bands too large on agarose gel
Started by cyclicstrawberry, Feb 24 2015 08:20 AM
pcr agrose gel electrophoresis
1 reply to this topic
#2
phage434
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Posted 24 February 2015 - 12:18 PM
I'm guessing you have overloaded the gel, and have too much DNA in the band to make a sharp band. Try diluting 10x to 100x and trying again (or loading diluted samples in different lanes).
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