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PCR DNA bands too large on agarose gel

pcr agrose gel electrophoresis

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#1 cyclicstrawberry

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Posted 24 February 2015 - 08:20 AM

Hello,

 

I have carried out gel electrophoresis for a construct that should be ~800bp. However, the DNA band spans from 750-950bp, instead of the usual thin line as the band. Why has this happened?

 

Thanks.



#2 phage434

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Posted 24 February 2015 - 12:18 PM

I'm guessing you have overloaded the gel, and have too much DNA in the band to make a sharp band. Try diluting 10x to 100x and trying again (or loading diluted samples in different lanes).







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