Hey, I want to study lung morphology as well as perform IHC staining in asthmatic condition.
If morphological structure is secured...I am planning to fix the lungs by Perfusion (using .1% PBS to wash away all blood and then to fix it with 4% freshly prepared Paraformaldehyde solution) before keeping in dry ice-Isopentane slurry.
If there is any available protool for the ame, please let me know
