Hi BioForum people.
I have a question about an autoclave issue I just encountered. The protocol I have states that my pre heat (before sterilization) and cooling has to have a certain amount of time, but when I was looking at my autoclave print out I could see that the time for both the pre heat and cooling was 2 minutes quicker than the protocol said. That made me realize that I dont really understand that requirement in my protocol, could anyone explain to me what kind of reasoning this could have? And if I need to repeat my experiment? The only thing I can really thing of is that I dont want a to quick heat up because their could be some sort of over boiling of the liquid, but otherwise I am not sure. Hope there are some people out there with more autoclave knowledge than me