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Erythromycin selection of transformed e.coli

erythromycin antibiotic selection e.coli transformation cloning

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#1 Meg P. Anula

Meg P. Anula

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Posted 18 February 2015 - 03:54 AM

Hi, does anyone here has experience on antibiotic selection using erythromycin?

 

Care to give me some advice?

 

I've been having problem with the right concentration of erythromycin to select among the e.coli strain with recombinant plasmid.

100 ug/ml seems to be too low as it introduces either too many background or false positive growth.

 

And I've heard people told me about plating from lower to higher concentration (instead of jumping right to high concentration) to ensure successful growth of transformed colonies. 

 

Any advices/opinions will be highly appreciated.







Also tagged with one or more of these keywords: erythromycin, antibiotic selection, e.coli, transformation, cloning

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