I was wondering what a good time point is for performing cell cycle analysis after transiently transfecting human cells or dox induction of a stable cell line? This is in reference to overexpression of a transcription factor that regulates genes involved in cell cycle. Would 72hrs be sufficient to see alterations in cell cycle?
Trying to determine the optimal endpoint from overexpression until cell cycle harvest from steps 1-3: 72hrs?
1. Overexpress gene of interest/functional protein
2. Transactivation of targets genes involved in cell cycle regulation
3. Cell cycle analysis for alterations.
Just now sure how many hours are needed to see this effect?