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phosphatase inhibitors


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#1 zingmatter

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Posted 25 July 2004 - 11:51 PM

I want to do phospho-IP from mammalian cell extracts and am trying to select phospahtase inhibitors while avoiding anything too toxic (if possible).

Any have any ideas or experience with this?

Thanks

#2 Sprag

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Posted 03 August 2004 - 08:08 AM

You can use Caliculin A for Serine/theronine phosphatases and sodium vanadate for Tyrosine phosphostases.

Just add these to your sample buffer when lysing your cells.




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