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Fibronectin Purification


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#1 anonymous

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Posted 28 June 2001 - 09:00 PM

Hello..

Could you give me some comment about Fibronectin Purification process?

After first elution using 4M urea, for second gelatin column, how can I eliminate urea?

dialysis or prewashing gelatin column with 4M urea is right?



#2 anonymous

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Posted 09 July 2001 - 09:00 PM

dialysis will be fine, make sure you add a protease blocker in your dialysis buffer




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