I am working on neurons. Today I was wrong with the dilution of Triton X100 to permeabilize the cells. I should prepare 0.1% Triton X 100 but I realized that I prepared 0.8% Triton X 100. My staining is on MAP2 and on synaptic markers (synaptophisin, PSD-95, VGLUT1, VGAT, Gephyrin)
How much this mistake affects the experiment?