Just starting on apoptosis!!!
I observed that in normal keratinocytes and treated keratinocytes that there are diferente levels of Bcl-2.
I am willing to better understand this finding and want to analyse apoptosis of these cells.
How do I define which concentration of ABT-737 I should use in my cells to induce apoptosis? How long do I treat the cells with ABT-737 before anexin V analysis?
I will be very happy if someone could advice me.