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ABT-737 induction of apoptosis


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#1 vecttoraz

vecttoraz

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Posted 10 February 2015 - 10:44 AM

blink.png Dear All,

 

Just starting on apoptosis!!!

 

I observed that in normal keratinocytes and treated keratinocytes that there are diferente levels of Bcl-2.

 

I am willing to better understand this finding and want to analyse apoptosis of these cells.

 

How do I define which concentration of ABT-737 I should use in my cells to induce apoptosis? How long do I treat the cells with ABT-737 before anexin V analysis?

 

I will be very happy if someone could advice me.

best

 

Vecttoraz






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