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ABT-737 induction of apoptosis

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#1 vecttoraz



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Posted 10 February 2015 - 10:44 AM

blink.png Dear All,


Just starting on apoptosis!!!


I observed that in normal keratinocytes and treated keratinocytes that there are diferente levels of Bcl-2.


I am willing to better understand this finding and want to analyse apoptosis of these cells.


How do I define which concentration of ABT-737 I should use in my cells to induce apoptosis? How long do I treat the cells with ABT-737 before anexin V analysis?


I will be very happy if someone could advice me.




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