member
Posted 09 February 2015 - 10:53 PM
hello everyone
Need help .
Cell suspension that I have =100ul of cell suspension with concentration 1x10^6 cells/ml
Concentration Antibody that I have (stock@purchased)= 200ug/ml
Concentration Antibody that have to be used in experiment= 1ug/1x10^6 cells
from supplier datasheet (Antibody): dilution= 1ug/1x10^6 cells.
How much volume I need to take out from the stock antibody to be used in my experiment?
Making glass out of shards
Posted 10 February 2015 - 04:54 AM
Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.
member
Posted 10 February 2015 - 05:38 AM
sorry, i m not understand. can u make it clear ?
Making glass out of shards
Posted 10 February 2015 - 07:28 AM
Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.
member
Posted 10 February 2015 - 04:05 PM
can you show me how to calculate?
member
Posted 10 February 2015 - 10:11 PM
100 ul of suspension that has 10_6 cells per 1000 ul contains 10_5 cells
1ug of antibody per 1000 ul so that means 0.1 ug for the 100 ul cells you have
your stock is 200 ug antibody per 1000 ul, and you just saw that you need 0.1 ug for the experiment...can you figure out the rest ?
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