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Antibody concentration


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5 replies to this topic

#1 hidayahwannur

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Posted 09 February 2015 - 10:53 PM

hello everyone

 

Need help .

 

Cell suspension that I have =100ul of cell suspension with concentration 1x10^6 cells/ml

Concentration Antibody that I have (stock@purchased)= 200ug/ml

 

Concentration Antibody that have to be used in experiment= 1ug/1x10^6 cells

 

from supplier datasheet (Antibody): dilution= 1ug/1x10^6 cells.

How much volume I need to take out from the stock antibody to be used in my experiment?



#2 Tabaluga

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Posted 10 February 2015 - 04:54 AM

100 ul of suspension that has 10_6 cells per 1000 ul contains 10_5 cells

1ug of antibody per 1000 ul so that means 0.1 ug for the 100 ul cells you have

your stock is 200 ug antibody per 1000 ul, and you just saw that you need 0.1 ug for the experiment...can you figure out the rest ?

Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 


#3 hidayahwannur

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Posted 10 February 2015 - 05:38 AM

sorry, i m not understand. can u make it clear ?blink.png



#4 Tabaluga

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Posted 10 February 2015 - 07:28 AM

which part don't you understand ?

Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 


#5 hidayahwannur

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Posted 10 February 2015 - 04:05 PM

can you show me how to calculate?



#6 hidayahwannur

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Posted 10 February 2015 - 10:11 PM

100 ul of suspension that has 10_6 cells per 1000 ul contains 10_5 cells

1ug of antibody per 1000 ul so that means 0.1 ug for the 100 ul cells you have

your stock is 200 ug antibody per 1000 ul, and you just saw that you need 0.1 ug for the experiment...can you figure out the rest ?






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