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Who can advise me a kit that would allow me to purify amplicons from quantitativ

QPCR

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3 replies to this topic

#1 mer

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Posted 09 February 2015 - 06:41 AM

I wish to sequence the bacterial DNA amplification products amplified by real-time quantitative pcr (SYBR Green method) for that I need a kit that would allow me to purify the amplified amplicons, so who can give me a name of a kit that would allow me to accomplish this task ? Thanks



#2 phage434

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Posted 09 February 2015 - 09:28 AM

Any normal pcr cleanup process will work for q-pcr cleanup. Nothing special is needed.



#3 memari

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Posted 09 February 2015 - 03:00 PM

I use this one form Favorgen that is the cheapest in market:

 

https://www.cedarlan...ck001&x=41&y=15

 

GEL/PCR Purification Mini Kit (300prep)

Code:
FAGCK001-1
Supplier:
Favorgen Biotech Corporation
Size:
300 Prep
 

BUY ONE GET ONE FREE: FAGCK001-1 AND FAPDE300
$141.00 CDN

Edited by memari, 09 February 2015 - 03:01 PM.

-----
Babak Memari

#4 phage434

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Posted 09 February 2015 - 04:12 PM

I've never heard of this company, but any kit of this kind will likely work. You could even likely just do an ethanol precipitation, which would be much cheaper.







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