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KE-37 cell culture conditions?


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#1 afraser

afraser

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Posted 09 February 2015 - 06:15 AM

Hi,

 

I am currently trying to grow up human KE-37 lymphoblastic cells to do centrosome isolation on.

 

I was using 500ml DMEM, 50ml FCS GOLD, 5ml pen/strep and 5ml L-Glut for my media. However the cells are not thriving and I have not been able to split them.

I have just switched to L-Glut free DMEM and removed the antibiotics and am instead using DMEM + FCS GOLD as an alternative.

 

Does anyone have experience with culturing KE-37 cells? And if so, can you make any suggestions as to what conditions I should change?

 

Thanks






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