I am currently trying to grow up human KE-37 lymphoblastic cells to do centrosome isolation on.
I was using 500ml DMEM, 50ml FCS GOLD, 5ml pen/strep and 5ml L-Glut for my media. However the cells are not thriving and I have not been able to split them.
I have just switched to L-Glut free DMEM and removed the antibiotics and am instead using DMEM + FCS GOLD as an alternative.
Does anyone have experience with culturing KE-37 cells? And if so, can you make any suggestions as to what conditions I should change?