Is it only through Western Blotting, there's no way I can tell if mRNA levels affected protein expression?
Edited by J092, 07 February 2015 - 04:10 PM.
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Protein knockdown method?
Started by J092, Feb 07 2015 04:05 PM
Best Answer bob1, 08 February 2015 - 12:26 AM
WB is the standard, you could also use immunocytochemistry or any other discriminatory protein detection method. WB is semi-quantitative (given the right conditions fully quantitative) and quick and cheap which are the main advantages.
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Posted 07 February 2015 - 04:05 PM
I performed siRNA transfection on cells monitoring mRNA expression, how would I relate this to determining a change in protein expression?
Is it only through Western Blotting, there's no way I can tell if mRNA levels affected protein expression?
Is it only through Western Blotting, there's no way I can tell if mRNA levels affected protein expression?
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Posted 08 February 2015 - 12:26 AM Best Answer
WB is the standard, you could also use immunocytochemistry or any other discriminatory protein detection method. WB is semi-quantitative (given the right conditions fully quantitative) and quick and cheap which are the main advantages.
