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Protein knockdown method?


Best Answer bob1, 08 February 2015 - 12:26 AM

WB is the standard, you could also use immunocytochemistry or any other discriminatory protein detection method.  WB is semi-quantitative (given the right conditions fully quantitative) and quick and cheap which are the main advantages.

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#1 J092

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Posted 07 February 2015 - 04:05 PM

I performed siRNA transfection on cells monitoring mRNA expression, how would I relate this to determining a change in protein expression?

Is it only through Western Blotting, there's no way I can tell if mRNA levels affected protein expression?

Edited by J092, 07 February 2015 - 04:10 PM.


#2 bob1

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Posted 08 February 2015 - 12:26 AM   Best Answer

WB is the standard, you could also use immunocytochemistry or any other discriminatory protein detection method.  WB is semi-quantitative (given the right conditions fully quantitative) and quick and cheap which are the main advantages.






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