What is it exactly that you need to know?
For example: in some strains you can already see that the his3 gene has been mutated by putting another gene in it.. so the strain is auxotrophic for histidine...
Sorry for not responding - i had given up on the thread!
What i need is basically an idea of what the different "signs/nomenclature" mean.
Like, :: and : and some kind of overview on how mutations are generally written down - general rules/guidelines/something. It's like it's written in a language that i don't understand.
It's difficult to tell (for me) whether or not the mutation "srs2::HIS3" means that the srs2-gene has ben inserted into HIS3, or that the HIS3 gene has been inserted into the srs2 gene for example.
I know Delta means the gene has been deleted - so i'm guessing "ade2-n(deltaNdel)" means a deletion of the ade2-n gene, but i'm not sure.
LYS2 means... what? A mutation in the LYS-biopathway, or is it just to signify WT LYS? Would LYS2 make a strain auxotrophic for lysine?
Why sometimes the capital letters and sometimes not?
Like "LYS2" vs. "lys2::GAL-IsceI" - both include lys2, but differ in the way it's written. And i'm not sure what the "lys2::GAL-IsceI" even means, too.
Anyhow, it's all just terribly confusing to me
Thanks in advance for your help.
This might already help (I have not a lot of time now):
(most important link is at at the bottom btw, in my explanation)
Just check for the parts dealing with nomenclature.
To answer your questions quickly: the :: means that the gene in front of the :: has been partly replaced by the gene behind the ::
so X::Y means that Y was inserted in gene X
delta does indeed mean deleted.
LYS2 just means its there and depending on how its written its the wild type or not or... (see link below)
Check for sure this : https://www.unifr.ch...astGenetics.pdf
download this book ! Its a very good guide! and check the part on the nomenclature, it will already help a lot!!!
(together with this: http://wiki.yeastgen...y_used_strains)