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mRNA of bacteria


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#1 weimin42

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Posted 25 July 2004 - 08:06 PM

It is well known that the mRNAs of bacteria are very short and unstable. anyone there know where i can get one perfect mRNA isolation procedure . Another question is about the prepare of cDNA probe with 33P for macroarray. would anyone give me some suggestions on this topic.

Thanks in advance

weimin42

#2 postdoc

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Posted 25 July 2004 - 09:18 PM

Hi weimin,

I found a good guide from Ambion on the exact same topic

"Isolating Bacterial RNA for Array Analysis"
http://www.ambion.co...tn/91/9110.html

Hope it helps.




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