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polyclonal antibody vs monoclonal antibody


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#1 SF_HK

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Posted 05 February 2015 - 12:22 AM

Hi,

 

I'm studying a gene whose monoclonal antibody is not commercially available but polyclonal antibody is widely available.  I've seen a few papers use polyclonal antibody for IHC in a large sample size. I thought monoclonal antibodies are more specific and results are repeatable with such. Thought its nor advisable to conduct IHC using polyclonal antibodies.



#2 gfischer

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Posted 05 February 2015 - 08:34 AM

In theory monoclonal antibodies are better if you can get them, but staining should work fine with a polyclonal.  If you can use the same antibody as some of the published work, you should be fine.


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