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polyclonal antibody vs monoclonal antibody

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#1 SF_HK



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Posted 05 February 2015 - 12:22 AM



I'm studying a gene whose monoclonal antibody is not commercially available but polyclonal antibody is widely available.  I've seen a few papers use polyclonal antibody for IHC in a large sample size. I thought monoclonal antibodies are more specific and results are repeatable with such. Thought its nor advisable to conduct IHC using polyclonal antibodies.

#2 gfischer



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Posted 05 February 2015 - 08:34 AM

In theory monoclonal antibodies are better if you can get them, but staining should work fine with a polyclonal.  If you can use the same antibody as some of the published work, you should be fine.

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