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Different drug treating time

MTT assay

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3 replies to this topic

#1 cell 293

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Posted 04 February 2015 - 11:45 PM

Hi, everyone

 

To determine cell viability with MTT assay, many time points (6, 12, 24, 48 hours) with drug treatment are detected.

 

What is meaning of different time with drug treatment? 

 

 

Thanks a lot



#2 bob1

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Posted 05 February 2015 - 01:03 PM

how quickly does the drug work... do you expect the death to be instantaneous?



#3 cell 293

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Posted 05 February 2015 - 07:46 PM

I don't know how to choice the time point for testing cytotoxicity of drug.

 

Do i just choice the time point that i can see dose-dependent?

 

Can different time point represent the acute or chronic toxicity?



#4 bob1

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Posted 07 February 2015 - 12:29 AM

If you don't know these details then  you need to do a range of concentrations in a time-course experiment... that's what the time series are for.







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