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Can I skip the FC blocking step in flow cytometry?

fc block fc blocking reagent

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3 replies to this topic

#1 H. Aly

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Posted 03 February 2015 - 11:11 AM

Can I skip the FC blocking step in flow cytometry?  and when I shouldn't do that?



#2 Tabaluga

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Posted 03 February 2015 - 04:21 PM

You can skip it but it might lead to a higher background. I wouldn't do it when staining macrophages or monocytes since they have a lot of Fc receptors


Edited by Tabaluga, 03 February 2015 - 04:22 PM.

Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 


#3 CPRES

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Posted 03 February 2015 - 05:55 PM

As Tab said, however, you can do away this step if you are staining whole blood (because there is a lot of serum in the whole blood blocking the Fc receptors).


So. Now that you have your first ever question on bioforum answered (or not), mail yourself your username and password so you don't forget them, and then come back soon to update us on how it all worked out. That's how you build Karma in science.


#4 H. Aly

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Posted 04 February 2015 - 12:50 PM

what about staining a monolayer cell line not blood ?







Also tagged with one or more of these keywords: fc, block, fc blocking reagent

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