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Transformation E.Coli help


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#1 labgirl14

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Posted 03 February 2015 - 10:49 AM

Hi, I just did a transformation but I accidentally added the luria broth before heatshocking...would my E.Coli have taken up the plasmids? I plated them anyway and will know the answer tomorrow morning but wanted to know what to expect!

Thank you

#2 CPRES

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Posted 03 February 2015 - 05:50 PM

No. Please update us on how profound was your disappointment when you opened the incubator tomorrow :)


So. Now that you have your first ever question on bioforum answered (or not), mail yourself your username and password so you don't forget them, and then come back soon to update us on how it all worked out. That's how you build Karma in science.


#3 labgirl14

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Posted 03 February 2015 - 07:44 PM

Oh no....I knew it inside but I was holding on haha. I'm just curious but could you explain why adding lb before heatshock is bad? I get what heat shock does, but would the lb dilute my competent ecoli or something?




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