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is -40 freezer enough for cell freezing?

cell freezing protocol

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#1 KKK



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Posted 02 February 2015 - 11:28 PM

I have routinely used the standard IPA container in -80 overnight for cell freezing before transferring it into liquid nitrogen. But at the new place I have joined, we have only a -40 feeder and no -80 freezer. Can I replace -40 with -80 during freezing before liquid nitrogen step? How important it is to take cells to -80 before immersing in liquid nitrogen?









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Posted 03 February 2015 - 06:10 PM

Yes, you can transfer from -40 to LN2 without problem. I have done it several times with several cell lines, out of impatience, so I am sure it works.


However, don't do it with some sensitive or irreplaceable cell line without doing some trial runs. And if you do, don't sue me. What's with your Avatar name by the way?

So. Now that you have your first ever question on bioforum answered (or not), mail yourself your username and password so you don't forget them, and then come back soon to update us on how it all worked out. That's how you build Karma in science.

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