I am going to do some ELISpot assays and I have obtained cryopreserved PBMCs from a commercial source.
However, the number of cells in each vial is very high and much more than what I would need for each set of experiment.
Rather than wasting the extra PBMCs, I am wondering if it is possible to cryopreserve them again after a short period of culture. Would it affect the immune functions of the different cell subsets?
I would really appreciate some advice on this.