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Sirna transfection DNA contamination

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#1 J092



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Posted 28 January 2015 - 01:48 PM

I've done sirna transfection on my cells but when run the products on an electrophoresis gel it was shown to be highly contaminated with DNA, they were treated with DNase I, I then did cDNA and checked if silencing worked using qRT-PCR, it did not. However I had kept the conditions in which the first time it had worked with other samples. Is the DNA contamination a possible reason for it not working even if treated with DNase I?

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