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Is it possible to "over-block" a membrane?


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5 replies to this topic

#1 science noob

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Posted 28 January 2015 - 04:49 AM

Most protocols suggest blocking at RT for 1 hour. But would leaving it a few hours longer cause anything detrimental to the final outcome of the Western?



#2 mdfenko

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Posted 28 January 2015 - 05:44 AM

it shouldn't, as long as your block is stable (no reason why it shouldn't be).

 

if you have concerns then you can block at 4C (usually overnight)


talent does what it can
genius does what it must
i used to do what i got paid to do


#3 neuron

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Posted 30 January 2015 - 01:58 AM

Yes I agree with mdfenko, we do at 4 degrees for overnight.



#4 CPRES

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Posted 30 January 2015 - 06:11 AM

Wine, Whiskey and Western block, all improve with time.


So. Now that you have your first ever question on bioforum answered (or not), mail yourself your username and password so you don't forget them, and then come back soon to update us on how it all worked out. That's how you build Karma in science.


#5 mdfenko

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Posted 30 January 2015 - 06:20 AM

sometimes wine sours


talent does what it can
genius does what it must
i used to do what i got paid to do


#6 CPRES

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Posted 30 January 2015 - 06:26 AM

Wine is more like women, not all improve with age, but some do. Whiskey is more like men, all of them mellow with age.


So. Now that you have your first ever question on bioforum answered (or not), mail yourself your username and password so you don't forget them, and then come back soon to update us on how it all worked out. That's how you build Karma in science.





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