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Ab titration


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#1 Iceage

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Posted 23 January 2015 - 06:15 PM

How important is it to titrate antibodies for human blood? I have done a lot of flow stainings in mouse cells, and always titrated abs. But with human cells, due to limited numbers of PBMCs, was wondering if I could just rely on what the company recommends. Also, I am doing stainings with much lower numbers of cells/tube (~25-50,000). Should I lower the Ab concentration? Any help of suggestions are greatly appreciated!

#2 CPRES

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Posted 25 January 2015 - 02:04 PM

Recommended antibody titer for any particular cell does not change with cell numbers, unless there are way too many of them. So, yes, rely on the company recommendation.


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