I ve been doing retroviral transductions on t cells for awhile now and while i get transduction efficiencies of 50% or more, the results i get from the experiments i set up with the transduced cells vary from experiment to experiment.
To be more specific, i analyze the effects of over expressing a protein on t cell proliferation. most of the time it reduces proliferation but of late it doesnt do anything to proliferation. I know its not because of a mutation in the vector or lack of protein expression. In my western blots, its obvious the protein is clearly over expressed.
Any thoughts on what could be going on here would be appreciated!