Hi, I work with primary hepatocytes - these cells are post-mitotic so the cells can only be used once. The cryo preserved cells are quite expensive and often the vial contains more cells than I need. Once I thaw a vial and take out a fraction, can the remaining cells be frozen back? The media used to freeze is propriety - just adding DMSO will not work. I will try this the next time I use cells - I just thought I'd ask in case someone here has thoughts or experience.