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antibody/DNAconjugation and purification

antibody oligo conjugation purification

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#1 Deepblue

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Posted 17 January 2015 - 04:25 PM

Hi,

 

I need to attach a single strand DNA oligo to an antibody molecule. Any good suggestions on the strategy? I know there are some kits available. But I want to hear from those who have experiences which method is the best in terms of efficiency and easiness of use. After conjugation, the DNA oligos need to be removed. Any suggestions on that too? The DNA oligo is about 18kD (60 nucleiotide). Size exclusion chromatography would be an option. But I cannot find a column that can deal with a small volume (100 ul).

 

Thanks.



#2 CPRES

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Posted 18 January 2015 - 04:07 PM

Haven't done this, but read the first link here (solulink), and also look up the references in that paper for some of the questions you have posed. Best.

 

Of course, within a few days you will be an expert in this, so please come back and tell us how it is done.


Edited by CPRES, 18 January 2015 - 04:08 PM.

So. Now that you have your first ever question on bioforum answered (or not), mail yourself your username and password so you don't forget them, and then come back soon to update us on how it all worked out. That's how you build Karma in science.






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