Hi all, I've some problem with my transformation. When I did antibiotic selection, both transformants (e.coli with plasmid of interest) and control (without plasmid) showed growth.
I picked a few colonies from the transformant plate anyway and growed in broth with 100ug/ml erythromycin overnight. Then I extracted the plasmid and run agarose gel and the results showed band around the similar bp of the plasmid of interest.
Since the antibiotic selection failed (I doubt if the antibiotic concentration is too low), I cannot be entirely sure the plasmid transformation was successful.
So my question is, based on the agarose results, can I be sure the plasmid extracted is indeed the plasmid I want? Or could it be the naturally occuring plasmid in e.coli strain w3110IQ instead?
Pls, if anyone has any idea, kindly advice. Thanks.