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Research topic related to jurkat cell line


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#1 Allan Chan

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Posted 16 January 2015 - 12:45 AM

Hi all,

I am an undergraduate student doing my internship in this semester. Can anybody suggest me that what are the good research titles related to jurkat cell line? Previous intern did optimal seeding density on jurkat cells. So can anyone please give some advice on that? By the way, the duration of my attachment is 5months. Thank you in advance. 



#2 CPRES

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Posted 16 January 2015 - 07:36 AM

This depends entirely upon what your mentor wants you to accomplish in 5 months, how much resources she can spare for you, and what is your level of enthusiasm for learning and achieving something.

 

Jurkat cell line has been around since before I was born (which was not last year), so while you can do things like optimal seeding density for months on end, if I were in your place, I would say what is the point. But that is me, I was more ambitious even as a undergraduate student.

 

Tell you mentor that you will give it your all, that you are willing to learn and work hard for it, and ask for a worthwhile project that only she can think of in the context of her resources and research direction. 

 

Sorry, no definitive help, but you may thank me later.


So. Now that you have your first ever question on bioforum answered (or not), mail yourself your username and password so you don't forget them, and then come back soon to update us on how it all worked out. That's how you build Karma in science.


#3 Allan Chan

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Posted 22 January 2015 - 04:31 PM

Hi,
i saw ur reply to the topic that i have posted. Actually the company that i am doing my internship now is trying to develop bio-reactor for activated cytotoxic T lymphocytes. Since jurkat cells are T lymphocytes, they are trying to use that to mimic CTL. Currently they are facing the problem with the activated T lymphocyte. Once activated, the viability of the lymphocytes decrease but we don't know the certain reason behind. There is a suggestion that this may be due to the shear stress during the pipetting while stimulation. So how can we increase the viability of the cells?
Sorry if my question is a bit long-winded and complicated.



#4 animodel123

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Posted 25 February 2015 - 06:44 PM

For your reference, here are some researches related to Jurkat cells: Electroporation of jurkat cells with siRNA Docosahexaenoic acid induces apoptosis in Jurkat cells by a protein phosphatase-mediated process The natural tyrosine kinase inhibitor genistein produces cell cycle arrest and apoptosis in Jurkat T-leukemia cells Presence of a pre‐apoptotic complex of pro‐caspase‐3, Hsp60 and Hsp10 in the mitochondrial fraction of Jurkat cells Sequential activation of three distinct ICE-like activities in Fas-ligated Jurkat cells Activation of intrinsic and extrinsic pathways in apoptotic signaling during UV-C-induced death of Jurkat cells: the role of caspase inhibition L-Arginine regulates the expression of the T-cell receptor ζ chain (CD3ζ) in Jurkat cells α-Lipoic acid increases intracellular glutathione in a human T-lymphocyte Jurkat cell line




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