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medium Tissue culture

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#1 Homeostasis

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Posted 14 January 2015 - 01:10 PM

In general, about how long can you leave your medium in a water bath (37*C) before it begins to go bad?


Edited by Imathomeostasis, 14 January 2015 - 01:11 PM.


#2 CPRES

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Posted 14 January 2015 - 01:25 PM

That you can only find out by a series of time-course experiments using dozens of media tested on hundreds of cell lines and checking the cells not only for death, but microarray expression analysis. Meaning, it is difficult to say.

 

The culture media are complex solutions; each ingredient with its own half-life and sensitivity to temperature. With that said, you keep cells in culture at 37'C with the same media for 2-4 days, so just try not to leave them too long in water bath (couple of days at the most). If you do keep it in water bath for more than a few hours, go ahead and label it with this information, and use it quickly on the cells which don't have a crucial experiment coming up (you will be fine with a medium left for 2 weeks at 37' in that case).


Edited by CPRES, 14 January 2015 - 01:26 PM.

So. Now that you have your first ever question on bioforum answered (or not), mail yourself your username and password so you don't forget them, and then come back soon to update us on how it all worked out. That's how you build Karma in science.


#3 Homeostasis

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Posted 14 January 2015 - 01:39 PM

That you can only find out by a series of time-course experiments using dozens of media tested on hundreds of cell lines and checking the cells not only for death, but microarray expression analysis. Meaning, it is difficult to say.

 

The culture media are complex solutions; each ingredient with its own half-life and sensitivity to temperature. With that said, you keep cells in culture at 37'C with the same media for 2-4 days, so just try not to leave them too long in water bath (couple of days at the most). If you do keep it in water bath for more than a few hours, go ahead and label it with this information, and use it quickly on the cells which don't have a crucial experiment coming up (you will be fine with a medium left for 2 weeks at 37' in that case).

 

Very helpful, thank you!







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