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"Secret" Ingredient in Potato Dextrose Agar (PDA)?

Started by Labmate0910, Jan 14 2015 12:22 PM

PDA potato dextrose agar microbiology phytopathology pathology

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1 reply to this topic

#1 Labmate0910

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Posted 14 January 2015 - 12:22 PM

Hello,

I've heard a few rumors from companies that there is more to PDA than just potato infusion, dextrose (glucose), and agar. When we prepare it in our lab and compare to supplier bought PDA, performance of our lab-made medium is less. Has anyone heard of this rumor, or possibly has suggestions on how you make your PDA?

We currently make it using:

Glucose: 20g

Potato infusion powder: 4 g

Agar: 15g

Thanks in advance for any advice!

#2 CPRES

Cellcounter

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140 posts

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Posted 14 January 2015 - 01:03 PM

No secret. They use potatoes from the best of family farms in Idaho!

Joking aside, that's why people buy stuff from companies. The process standardization, consistency, etc.

The secret ingredient may be perfect pH too. When did you last calibrate your meter?

So. Now that you have your first ever question on bioforum answered (or not), mail yourself your username and password so you don't forget them, and then come back soon to update us on how it all worked out. That's how you build Karma in science.

Back to Tissue and Cell Culture

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