Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Na2Hpo4 , NaH2po4 or both in Church buffer ?!


  • Please log in to reply
2 replies to this topic

#1 mybioweb2

mybioweb2

    member

  • Active Members
  • Pip
  • 8 posts
0
Neutral

Posted 22 July 2004 - 02:28 PM

hello all

Does anybody know if it's absolutly necessary to use both Na2Hpo4 and NaH2po4 to make sodium phosphate solution for Church buffer preparation or i could simply use 0.5 M Na2Hpo4 only (without NaH2po4) to do Church buffer ? there are many different recipes for church buffer which make me confusing and perplexed !!

thankx very much

#2 postdoc

postdoc

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 52 posts
1
Neutral

Posted 22 July 2004 - 06:35 PM

Yes, you need both. check this protocol
http://www.molgen.mp...ol/09/09_06.doc

#3 mybioweb2

mybioweb2

    member

  • Active Members
  • Pip
  • 8 posts
0
Neutral

Posted 23 July 2004 - 04:59 AM

OK , thanx a lot. But why we need the two sodium phosphate solutions if we can obtain the 0.5 M from Na2Hpo4 or NaH2po4 only ?

Edited by mybioweb2, 23 July 2004 - 05:00 AM.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.