I've some contamination on my whole protein extracts. my protein of interest is a transcription factor.
I used Hek cells , 90 % confluent, RIPA buffer ( tris 50mM, Nacl 150mM, EDTA 5mM, NP-40 10%, Na deoxycholate 0.5% , SDS 0.1% and water .
On my blots , i've a lot of bands, either with a validate antibody GFP. Do you have any idea about the problem ? should i change my lysis buffer , or avoid sonication ? or other ideas are more than welcome.