I am at present working with antibiotic screening on cardiac cells, which results in very few number of cells after treatment.
With this low number of cells, western blot and RNA extraction becomes difficult.
Please give me an idea about downstream processes or assays which i can utilize to study signaling pathways in very few number of cells.
Does there exists any kits or array to give results from very few number of cells? immunocytochemistry is the only method which i could find so far.
Please please help
Thank you in advance