I've been having a bit of difficulty finding out information about the EM7 promoter. So far the only info I've found is that the EM7 promoter is a synthetic modification of the bacteriophage T7 promoter. Does anyone else know anything more about this promoter and why it is used?
The plasmid I'm working with has an EM7 promoter which drives expression of the blasticidin resistance gene to allow for selection in mammalian cells. However, we are having issues generating stable cell lines and are wondering if promoter competition is occurring with the CMV promoter.