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Affinity Purification of 5′ Capped mRNA by Using High-Affinity GST-4E

mRNA RNA PROTEIN EIF4E

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#1 AshChin

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Posted 04 January 2015 - 10:06 AM

Hi,

 

Does anyone have experience with purification of 5′ Capped mRNA by Using High-Affinity GST-4E.

Also does protein contamination inflate RNA concentration on the nano drop?

 

 



#2 CPRES

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Posted 13 January 2015 - 10:36 AM

Answer to your second question: YES


So. Now that you have your first ever question on bioforum answered (or not), mail yourself your username and password so you don't forget them, and then come back soon to update us on how it all worked out. That's how you build Karma in science.






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