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How to fully identify the virus?

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#1 Adam0



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Posted 03 January 2015 - 11:28 AM

Hello, I have small request for you.


Let's wonder the situation that some new virus has been discovered recently. The problem is that, we don't know nothing about it.


Could you hint me, what experimantal methods will be best to fully recognize this virus?


I mean methods that can give answers like:


Gonem size and type:RNA or DNA, next: ssRNA, ssDNA, dsRNA, dsDNA?

How to magnify genome[for sequencing] if we don't know sequence  [impossible to make primers for PCR ]?

If amplification is done, what method will be best for sequencing, Sanger, NGS, [RNAseq id RNA genome]?

How to get some information about viral proteins and their interaction in the cell?


Looking forward to your answers :)


#2 Trof


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Posted 04 January 2015 - 09:25 AM

Welcome to the forums,


as we are being in a homework forum, we are looking forward to your suggestions first.


Try solve it yourself first and show us what you got, others then may point you to the things you may have missed. 


How would you answer those questions?

Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.

I never trust anything that can't be doubted.

'Normal' is a dryer setting. - Elizabeth Moon

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