Hello, I have small request for you.
Let's wonder the situation that some new virus has been discovered recently. The problem is that, we don't know nothing about it.
Could you hint me, what experimantal methods will be best to fully recognize this virus?
I mean methods that can give answers like:
Gonem size and type:RNA or DNA, next: ssRNA, ssDNA, dsRNA, dsDNA?
How to magnify genome[for sequencing] if we don't know sequence [impossible to make primers for PCR ]?
If amplification is done, what method will be best for sequencing, Sanger, NGS, [RNAseq id RNA genome]?
How to get some information about viral proteins and their interaction in the cell?
Looking forward to your answers