Hi, our lab has a problem which studies the properties of DNA polymerases, and we are trying to identify an extremely rare mutation (deletion/insertion) that current sequencing technologies cannot detect with accuracy (i.e. the error is higher than the signal we're looking for) so i was wondering if we could use separation by capillary electrophoresis. Here's my rationale, capillary electrophoresis is used in sanger sequencing and thus is able to separate molecules that are 1 bp longer or shorter than each other, however, sanger sequencing is not an option for us give the huge cost that would involve. I'm looking to see if a service where capillary electrophoresis is detached from sanger sequencing and can work for us as an alternative for running a gel and for sequencing.
Edited by Raygoza, 01 January 2015 - 02:57 AM.