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Propagation Isolation - Current Lab Techniques Question

propagation isolation culture

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#1 daBee

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Posted 31 December 2014 - 02:38 AM

Hi folks.  I have a specific application in microbiology that I am investigating.  I found this forum so I joined so that I could put it forward.  Hopefully someone has some insight.

 

I'm looking for a way to separate filial generations in a microbiological propagation environment.  I'm searching for a way to follow the maximum F(x) through isolation and propagation.  

 

The issue is a bit fuzzy.  Bacterial (or Fungi) generations result from division.  Daughter cells are obviously children, but are in the same culture media as the parent cell.  I need to have a way of separating those.  

 

It's been some time since I've studied lab techniques and thought there might be a new way to do this.  The reason I need this is to avoid being called out on the final results being derived from what could possibly be argued, as from the F0 generation, seeing as they are not removed.  

 

Yeast or bacteria.  

 

Any insight appreciated.  

 

Cheers



#2 phage434

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Posted 31 December 2014 - 04:19 AM

For yeast, this is a well established technique. See:

Helmstetter CE. Description of a baby machine for Saccharomyces cerevisiae.
New Biol. 1991 Nov;3(11):1089-96. PubMed PMID: 1777482.
Grzelak A, Skierski J, Bartosz G. Decreased antioxidant defense during
replicative aging of the yeast Saccharomyces cerevisiae studied using the 'baby
machine' method. FEBS Lett. 2001 Mar 9;492(1-2):123-6. PubMed PMID: 11248249.

You may also want to look at the special purpose rotors for elutriation:

https://www.google.c....82001339,d.eXY



#3 daBee

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Posted 31 December 2014 - 04:27 AM

Ah very nice.  Would you know if this process has been automated, including sterilization?

 

Cheers



#4 phage434

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Posted 31 December 2014 - 04:30 AM

Not that I am aware of.



#5 daBee

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Posted 31 December 2014 - 04:31 AM

Thanks for the guidance.  Two options I can see.  

 

Cheers







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