I have a question about my PCR reaction,...
my original band is 78 bp( an aptamer)
when I run it ,instread of 78 bp band I have 120 bp I try to change Mg concentration and Tm but it couln't help
I test these one;
I aslo change my Primer concentartion but it doesn't help too
(my especific band is 78, 120 bp is nonespecific one)
please help me or say some sugestion