Dear all ,
I transfected a cell line with a shRNA plasmid. I did a transient transfection and after 72h (which is the peak of inhibition) I could not find a decrease in the target protein.
In parallel, I performed a transfection for stable selection of clones. I can now see 2 or 3 clones in the plate.
Is it worth keeping up with the selection of clones, since the transient transfection shows no downregulation of the target protein. Do you think the plasmid is working well?
Cheers and a very merry X-mas to all!